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  • 基因表达PCR芯片
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  • > 产品目录 > 科研产品 > 体细胞突变PCR芯片 > PI3K-AKT信号通路体细胞突变PCR芯片
    PI3K-AKT信号通路体细胞突变PCR芯片适用于快速、准确的进行PI3K-PTEN通路中常见突变位点的检测,包括:AKT1, AKT3, PIK3CA, PIK3R1, PIK3R5, PTEN和STK11/LKB1。PI3K-AKT通路基因突变已在人类多种肿瘤的发现(多于30%的实体瘤),因此,对该通路的深入研究有助于科研工作者更好的了解肿瘤的发生机制,并为临床用药提供可靠的分子机理。
    PI3K-AKT信号通路体细胞突变PCR芯片适用于快速、准确的进行PI3K-PTEN通路中常见突变位点的检测,包括:AKT1, AKT3, PIK3CA, PIK3R1, PIK3R5, PTEN和STK11/LKB1。PI3K-AKT通路基因突变已在人类多种肿瘤的发现(多于30%的实体瘤),因此,对该通路的深入研究有助于科研工作者更好的了解肿瘤的发生机制,并为临床用药提供可靠的分子机理。利用单个或多个体细胞突变信息检测关键信号通路异常已被广泛应用于临床研究。例如: EGFR和KRAS基因突变能够预测靶向该位点的药物治疗响应。PI3K-AKT通路体细胞突变PCR芯片覆盖广泛,适用于PI3K-AKT及其下游通路的突变检测,并有望为临床相关疾病提供潜在药物治疗靶点。该芯片包含PI3K-AKT通路中87个最常见并具有重要功能和生理学意义的突变位点。突变位点主要由多种体细胞突变数据库以及经同行评阅的科学文献筛选得到。芯片操作简单,只需借助real-time PCR仪即可完成,应用广泛。
     
    体细胞突变PCR芯片仅适用于分子生物学研究,不适用疾病的临床诊断及治疗。

     
    Assay functional annotations
    AKT genes:
    The mutation assays detects the best known AKT mutation, c.49G>A, p.E17K (AKT1; a PH domain mutation that results in constitutive targeting of AKT1 to plasma membrane), the activating mutations c.145G>A, p.E49K (AKT1) and c.511G>A, p.G171R (AKT3).
    PIK3CA (phosphatidylinositol 3-kinase catalytic subunit) gene:
    The mutation assays covered on this panel can detect 37 most frequently occurring PIK3CA mutations that are mainly clustered in the kinase domain (activating mutations) and in the P539-E545 region and vicinity, which are helical domain mutations that mimic activation by growth factors. The assays allow a comprehensive profiling of PIK3CA mutations that have been identified in a variety of cancers.
    PIK3R1 and PIK3R5 (PI3K regulatory subunit) genes:
    Two recurrent disinhibitory (c.1126G>A, p.G376R and c.1690A>G, p.N564D) mutations in PIK3R1 and a recurrent oncogenic mutation in PIK3R5 are included.
    PTEN gene:
    Included on the panel are 33 most commonly detected PTEN loss-of-function mutations that are mainly due to truncation or point mutation-caused phosphatase inactivation.
    STK11/LKB1 gene:
    Included on the panel are 11 most commonly detected STK11/LKB1 inactivation mutations that are mainly due to truncation or point mutation.

    规格:87个位点/array   1sample/array
    检测灵敏度:可以检测低至0.1%的体细胞突变


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