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  • > 产品目录 > 科研产品 > 体细胞突变PCR芯片 > 脑肿瘤体细胞突变PCR芯片
    人脑肿瘤(GBM/CNS)体细胞突变PCR芯片适用于快速、准确的进行GBM/CNS肿瘤相关基因突变的检测,包括:BRAF, CTNNB1/beta-catenin, EGFR, IDH1, IDH2, KRAS, NF2, NRAS, PIK3CA, 和PTEN。对上述基因突变的深入研究有助于科研工作者更好的了解肿瘤的发生机制,并为临床靶向用药提供可靠的分子机理。
    脑肿瘤体细胞突变PCR芯片
    人脑肿瘤(GBM/CNS)体细胞突变PCR芯片适用于快速、准确的进行GBM/CNS肿瘤相关基因突变的检测,包括:BRAF, CTNNB1/beta-catenin, EGFR, IDH1, IDH2, KRAS, NF2, NRAS, PIK3CA, 和PTEN。对上述基因突变的深入研究有助于科研工作者更好的了解肿瘤的发生机制,并为临床靶向用药提供可靠的分子机理。利用单个或多个体细胞突变信息检测关键信号通路异常已被广泛应用于临床研究。例如: EGFR和KRAS基因突变能够预测靶向该位点的药物治疗响应。人GBM/CNS肿瘤体细胞突变PCR芯片覆盖广泛,适用于GBM/CNS肿瘤以及含有此类突变的其他肿瘤的突变检测,并有望为临床相关疾病提供潜在药物治疗靶点。该芯片包含37个GBM/CNS肿瘤中最常见的,具有重要功能和生理学意义的突变位点。突变位点主要由多种体细胞突变数据库以及经同行评阅的科学文献筛选得到,展示了超过7800例GBM/CNS肿瘤患者(6850例神经胶质瘤)的最常见突变。
     
    Assay functional annotations
    BRAF: 1 Assay
    The most important BRAF mutation in brain cancer leads to increased kinase activity, the p. V600E mutation.
    CTNNB1: 5 Assays
    The most frequently detected CTNNB1/beta-catenin mutations result in abnormal signaling in the WNT signaling pathway. The mutated codons are mainly several serine/threonine residues targeted for phosphorylation by GSK-3beta.
    EGFR: 4 Assays
    The most frequently identified EGFR mutations include P-loop and activation loop point mutations, kinase domain deletions, and insertion mutations.
    IDH1: 5 Assays
    Most of these mutations abolish magnesium binding and alters the enzyme's activity to convert alpha-ketoglutarate into R(-)-2-hydroxyglutarate instead of isocitrate into alpha-ketoglutarate.
    IDH2: 3 Assays
    These mutations all lie in the substrate binding domain, and one (p.R140Q) is associated with D-2-hydroxyglutaric aciduria.
    KRAS: 2 Assays
    The mutation assays include the most frequently occurring mutations in KRAS codons 12, 13, and 61. Mutations at these positions result in reduced intrinsic GTPase activity and/or cause KRAS to become unresponsive to RasGAP.
    NF2: 1 Assay
    NF2 is similar to some members of the ERM (ezrin, radixin, moesin) family of proteins and links cell-surface proteins with cytoskeletal components and proteins involved in cytoskeletal dynamics. Mutations in this gene are associated with neurofibromatosis type II which is characterized by nervous system and skin tumors and ocular abnormalities.
    NRAS: 1 Assay
    The most important NRAS mutation in brain cancer occurs at codon 61.
    PIK3CA: 3 Assays
    The most frequently occurring PIK3CA mutations mainly belong to two classes: gain-of-function kinase domain activating mutations and helical domain mutations that mimic activation by growth factors.
    PTEN: 12 Assays
    The most commonly detected PTEN loss-of-function mutations are due to either truncation (p.R233* and p.R130*) or point mutations causing phosphatase inactivation (p.R130 and p.R173 mutations).
    规格:37个位点/array    2sample/96well array  8sample/384well array
    灵敏度:可以检测低至0.1%的体细胞突变


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