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  • 基因表达PCR芯片
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  • > 产品目录 > 科研产品 > 体细胞突变PCR芯片 > 结肠癌体细胞突变PCR芯片
    人结肠癌体细胞突变PCR芯片适用于快速、准确的检测人结肠癌最常见的基因突变,包括:APC, BRAF, CTNNB1/beta-catenin, FBXW7, KRAS, PIK3CA, SRC和P53。对上述基因突变的深入研究有助于科研工作者更好的了解肿瘤的发生机制,并为临床靶向用药提供可靠的分子机理。
    结肠癌体细胞突变PCR芯片
    人结肠癌体细胞突变PCR芯片适用于快速、准确的检测人结肠癌最常见的基因突变,包括:APC, BRAF, CTNNB1/beta-catenin, FBXW7, KRAS, PIK3CA, SRC和P53。对上述基因突变的深入研究有助于科研工作者更好的了解肿瘤的发生机制,并为临床靶向用药提供可靠的分子机理。利用单个或多个体细胞突变信息检测关键信号通路异常已被广泛应用于临床研究。例如: EGFR和KRAS基因突变能够预测靶向该位点的药物治疗响应。人结肠癌体细胞突变PCR芯片覆盖广泛,适用于结肠癌以及含有此类突变的其他肿瘤的突变检测,并有望为临床相关疾病提供潜在药物治疗靶点。该芯片包含86个结肠癌中最常见的,并具有重要功能和生理学意义的突变位点。突变位点主要由多种体细胞突变数据库以及经同行评阅的科学文献筛选得到,展示了超过29000例结肠癌患者的最常见突变。
     
    Assay functional annotations
    APC: 34 Assays
    The most commonly detected APC inactivation mutations are mainly composed of truncation mutations (due to nonsense mutations and frameshift mutations) and point mutations between codons 1250 and 1578.
    BRAF: 1 Assay
    In colon cancer, the BRAF mutation that leads to increased kinase activity, p. V600E mutation, is the most important to test.
    CTNNB1: 5 Assays
    The most frequently detected CTNNB1/beta-catenin mutations result in abnormal signaling in the WNT signaling pathway. The mutated codons are mainly several serine/threonine residues targeted for phosphorylation by GSK-3beta.
    FBXW7: 1 Assay
    The mutations queried by these assays lay in either the third or fourth repeat of the protein's WD40 domain, typically involved in protein-protein interactions.
    KRAS: 17 Assays
    The mutation assays include the most frequently occurring mutations in KRAS codons 12, 13, and 61. Mutations at these positions result in reduced intrinsic GTPase activity and/or cause KRAS to become unresponsive to RasGAP.
    PIK3CA: 7 Assays
    The most frequently occurring PIK3CA mutations mainly belong to two classes: gain-of-function kinase domain activating mutations and helical domain mutations that mimic activation by growth factors.
    SRC: 1 Assay
    SRC is a proto-oncogene and a tyrosine-protein kinase that plays a role in the regulation of embryonic development and cell growth. Mutations in this gene could be involved in the malignant progression of colon cancer.
    TP53: 20 Assays
    The most frequently detected somatic mutations in TP53 are largely composed of DNA-binding domain mutations which disrupt either DNA binding or protein structure.
    View a table of the mutations, associated COSMIC IDs and assay numbers, by clicking “Mutation Table” above on the right.
    规格:86个位点/array    1sample/array
    可以检测低至0.1%的体细胞突变


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